ESR 9 (Vrije Universiteit Brussel)

ESR9

Vrije Universiteit Brussel

Project Title:

Assessing the histological diversity of liver sinusoidal endothelial cells by optical nanoscopy

Objectives:

(1) SR-SIM assessment of the morphology and diversity of LSECs in different liver diseases (e.g. cancer, autoimmune disease, alcoholic and non-alcoholic fatty liver disease) by isolating cells from human donor material and mouse models of liver injury (e.g. liver fibrosis, non-alcoholic fatty liver disease, cancer)

(2) assess LSEC morphology and their interactions with other liver cells such as hepatic stellate cells and hepatocytes based on high-resolution imaging techniques (nanoscopy, EM) using immunohistochemistry of liver tissue and 3D hepatic spheroid cultures

(3) heterogeneity of LSECs isolated from diseased liver tissue assessed by correlative molecular analysis techniques and SR-SIM with extended z-resolution

Expected Results:

(1) LSECS from human and/or mouse pathological liver specimens isolated, fixed, and sorted based on specific liver disease

(2) morphology of isolated LSEC samples analyzed by SR-SIM

(3) morphology and cellular identity confirmed by SR-SIM within the consortium and correlated to electron microscopy

Universität Bielefeld - Biomolecular Photonics - Faculty of Physics - Universitätsstraße 25 - D-33615 Bielefeld - Germany

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