ESR 10 (LaVision BioTec)

ESR10

LaVision BioTec

Project Title:

Development of high-speed optical nanoscopy utilizing two-photon fluorescence excitation

Objectives:

(1) Integration of confocal two-photon fluorescence microscopy with rescanning SIM and image inversion interferometry

(2) utilize membrane deformable mirror optics to correct for wavefront distortions during deep tissue imaging

(3) multi-color imaging of liver sinusoids in fixed rodent tissue by 2-photon SR-SIM utilizing adaptive optics wavefront corrections

(4) high-speed 2-photon SR-SIM imaging of endothelial tubes, precision cut liver sections, and slices from histological preparations in perfusion chambers

Expected Results:

(1) Two-photon fluorescence microscopy with rescanning SIM and image inversion interferometry demonstrated (resolution 150 nm, speed: 8 frames/s)

(2) imaging of 3D structure of LSECs in planar and 3D cell culture in microfluidic chambers

(3) influence of small molecules (ethanol, paracetamol) and lipoprotein particles on fenestration stability imaged in perfused organ-on-chip models

Universität Bielefeld - Biomolecular Photonics - Faculty of Physics - Universitätsstraße 25 - D-33615 Bielefeld - Germany

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