ESR 2 (University of Oxford)

ESR2

University of Oxford

Project Title:

Adaptive optics 3D-AO-SIM for fast 3D super-resolution SIM imaging of fixed and live tissues

Objectives:

(1) Develop a low cost, portable SR-SIM solution

(2) Implement adaptive optics (developed at the Booth Lab, Oxford) to an upright SIM setup (Deep-SIM) developed at the Micron Oxford Advanced Bioimaging Unit

(3) Apply 3D-AO-SIM imaging to precision cut liver slices

(4) Image cells within tissue and their interaction with small molecules, nanoparticles in perfused, precision cut liver slices by 3D AO-SIM

Expected Results:

(1) Deep-SIM setup with adaptive optics and fast SLM pattern generation operational to enable aberration-free 3D-SIM imaging of fixed cells demonstrated (<150nm xy <400 nm z-resolution)

(2) high resolution and high speed SR-SIM imaging of 3D structure of LSECs in 3D cell culture in vitro demonstrated (1 3D fps)

(3) interactions of small molecules and nanoparticles with LSECs in perfused sinusoids demonstrated

Universität Bielefeld - Biomolecular Photonics - Faculty of Physics - Universitätsstraße 25 - D-33615 Bielefeld - Germany

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