Project 8 (University of Bielefeld)

Assessing signal transduction processes in LSECs under inflammatory conditions by optical nanoscopy

Project 8 - Eva Arias Bravo

University of Bielefeld

Objectives:

(1) SR-SIM investigation of signal transduction under inflammatory conditions using a transgenic mouse reporter for NF-kB to assess stress of isolated LSECs

(2) High-speed SR-SIM imaging to determine the effects of NF-kB inhibitors (Aspirin, glucorcorticoids), monitor all NF-kB DNA binding subunits (p50, p65, c-Rel, RelB and p52), and to follow inhibition of nuclear translocation of NF-kB by Cineol

(3) utilize combined 2-photon, high speed SR-SIM and microfluidic perfusion of liver slices to assess NF-kB inflammation markers in liver tissue under environmental control and environmental stress, i.e. hypoxia

Expected Results:

(1) LSECs and liver slices isolated from reporter mouse, NF-kB activation monitored by SR-SIM

(2) NF-kB inhibitor and activators in LSECs and liver slice cultures assessed

(3) distribution and dynamics of NF-kB DNA binding subunits in primary cells assessed

(4) 2-photon SR-SIM characterization of distribution of inflammation markers throughout perfused liver tissue completed

 

Project Lead:
Prof. Christian Kaltschmidt

Early Stage Researcher:
Eva Arias Bravo

 

Eva Arias completed a Bachelor’s degree in Biology from the Complutense University of Madrid, Spain. Later, she completed a Master´s degree in Microbiology at the University of Alcalá in Madrid, Spain. During the Bachelor’ and Master’s thesis, she worked with Strongyloides stercoralis, a human pathogenic parasite causing the disease strongyloidiasis.

Currently, Eva is the ESR number 8, and her project title is “Assessing Signal Transduction Processes in Liver Sinusoidal Endothelial Cells (LSECs) under Inflammatory Conditions by Optical Nanoscopy”. The main themes of her work are:

  • LSECs and liver slices isolated from reporter mouse, NF-κB activation monitored by SR-SIM.
  • NF-κB inhibitor and activators in LSECs and liver slice cultures assessed.
  • Distribution and dynamics of NF-κB DNA binding subunits in primary cells assessed.
  • 2-photon SR-SIM characterization of distribution of inflammation markers throughout perfused liver tissue completed.

 

 

 

Universität Bielefeld - Biomolecular Photonics - Faculty of Physics - Universitätsstraße 25 - D-33615 Bielefeld - Germany

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