ESR 14 (University of Tromsø)

ESR14

University of Tromsø

Project Title:

Super-resolution imaging of human LSECs in 3D culture and organ slices

Objectives:

(1) Optimisation of endothelial tube formation in 3D cell culture

(2) SR-SIM imaging of living LSECs forming endothelial tubes

(3) optimization of staining and SR-SIM imaging of living endothelial tubes, correlation to perfused liver slices in perfusion chambers

(4) SR-SIM and multifocal 2-photon SR-SIM imaging of endothelial cell interactions with small molecules and NPs in perfused sinusoids

Expected Results:

(1) Local supply of human LSECs established

(2) imaging of the 3D structure of LSECs in tubes and 3D cell culture in vitro completed

(3) high speed SR-SIM of human LSEC dynamics in 3D cell culture established

(4) SR-SIM imaging of interactions of small molecules and nanoparticles with LSECs in perfused sinusoids performed

Universität Bielefeld - Biomolecular Photonics - Faculty of Physics - Universitätsstraße 25 - D-33615 Bielefeld - Germany

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