Project 11 (University of Bielefeld)

Improving the optical resolution along the vertical direction for high speed 3D imaging of tissues

Project 11 - Cihang (Sherry) Kong

University of Bielefeld

Objectives:

(1) adaptation of high-speed GPU control and reconstruction routines for use with deep-tissue imaging by multi-focal SR-SIM

(2) Extension of high-speed SR-SIM to 50 nm spatial resolution by non-linear structured illumination (laterally) and combination with fluctuation analysis (axially)

(3) multi-color imaging of liver sinusoids in fixed rodent tissue by 2-photon fluorescence excitation based multifocal SIM

(4) High-speed multifocal SR-SIM imaging of perfused liver slices

Expected Results:

(1) Imaging of LSEC dynamics in vitro (100 nm res., speed 60 reconstructed frames/s)

(2) high resolution and high speed imaging of LSEC dynamics by nonlinear SIM (50 nm, 150 raw data fps)

(3) Super-resolution imaging assay for small molecules, i.e. ethanol, paracetamol; nanoparticles; in perfused tissue established, initial screening

 

Project Lead:
Prof. Thomas Huser

Early Stage Researcher:
Cihang (Sherry) Kong

Sherry completed her Bachelor of Engineering in Medical Engineering at the University of Hong Kong in 2015.

Her DeLIVER project will utilize high speed SR-SIM methods, including 2-photon excited fluorescence, in order to obtain isotropic super-resolved images of perfused liver sinusoids.

 

 

 

Universität Bielefeld - Biomolecular Photonics - Faculty of Physics - Universitätsstraße 25 - D-33615 Bielefeld - Germany

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