ESR 11 (University of Bielefeld)

ESR11

University of Bielefeld

Project Title:

Improving the optical resolution along the vertical direction for high speed 3D imaging of tissues

Objectives:

(1) adaptation of high-speed GPU control and reconstruction routines for use with deep-tissue imaging by multi-focal SR-SIM

(2) Extension of high-speed SR-SIM to 50 nm spatial resolution by non-linear structured illumination (laterally) and combination with fluctuation analysis (axially)

(3) multi-color imaging of liver sinusoids in fixed rodent tissue by 2-photon fluorescence excitation based multifocal SIM

(4) High-speed multifocal SR-SIM imaging of perfused liver slices

Expected Results:

(1) Imaging of LSEC dynamics in vitro (100 nm res., speed 60 reconstructed frames/s)

(2) high resolution and high speed imaging of LSEC dynamics by nonlinear SIM (50 nm, 150 raw data fps)

(3) Super-resolution imaging assay for small molecules, i.e. ethanol, paracetamol; nanoparticles; in perfused tissue established, initial screening

Universität Bielefeld - Biomolecular Photonics - Faculty of Physics - Universitätsstraße 25 - D-33615 Bielefeld - Germany

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