ESR 6 (University of Gothenburg)

ESR6

University of Gothenburg

Project Title:

Extending the versatility of optical nanoscopy by 3D optical force spectroscopy

Objectives:

(1) Development of 3D optical force spectroscopy of endothelial cells by calibration of polymer bead deviations by holographic optical tweezers near surfaces

(2) Integration of the force spectroscopy system with microfluidics and nanoscopy to monitor cellular response in vitro

(3) Determine changes in cellular fenestrations and the morphology of sieve plates in response to locally exerted forces

(4) Local enrichment of nanoparticle preparations by optical tweezers and cellular response in perfused liver sections

Expected Results:

(1) 3D optical force spectroscopy apparatus operational and calibrated

(2) mechanical properties of healthy, living LSEC membranes determined

(3) integration of nanoscopy with force spectroscopy system completed

(4) response of fenestrations and sieve plates to local, external forces determined

(5) changes in membrane elasticity due to exposure of LSECs to external agents and their reversibility determined in microfluidic cell culture

Universität Bielefeld - Biomolecular Photonics - Faculty of Physics - Universitätsstraße 25 - D-33615 Bielefeld - Germany

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