ESR 12 (University of Birmingham)

ESR12

University of Birmingham

Project Title:

Development of perfused human liver slice model for optical nanoscopy

Objectives:

(1) To develop a human precision cut liver slice model for imaging live human LSECs in normal and cirrhotic tissue specimens

(2) To adapt slicing system to permit intravascular perfusion of scavenger ligands and monoclonal antibodies, integration with organ-on-chip perfusion chambers

(3) To optimise culture and fixation protocols to permit temporal assessment of LSEC by high-speed SR-SIM imaging, 2-photon SIM imaging of perfused liver slices

Expected Results:

(1) methodology to permit dynamic visualization of cellular fenestrations in human LSEC and assess alterations by capillarisation and cirrhosis developed

(2) comprehensive assessment of the expression and subcellular localisation of scavenger receptors such as L-SIGN, Stabilin -1 and -2, CD36, Mannose receptor and FcR’s on human LSEC in situ

(3) detailed temporal analysis of the endocytic capabilities of human LSECs with respect to uptake of small molecules and lipoproteins

(4) optimised methodology for generation and culture of human precision cut liver slices for imaging modalities developed

Universität Bielefeld - Biomolecular Photonics - Faculty of Physics - Universitätsstraße 25 - D-33615 Bielefeld - Germany

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