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ESR4 Elvesys |
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Project Title: Development of 3D microfluidic cultivation and cell manipulation systems for optical nanoscopy |
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Objectives: Objectives: (1) Development of microfluidics platforms integrated with high-speed SR-SIM (2) Enabling controlled exposure of endothelial cells to small molecules and lipid nanoparticles while facilitating high speed, high resolution imaging of cellular morphology (3) Extension of microfluidics to facilitate LSECs endothelial tube formation replicating sinusoids in vitro in 3D cell culture and efficient fluorescent staining (4) Nanoscopy of fenestration dynamics in endothelial tube system |
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Expected Results: (1) High resolution imaging of LSEC dynamics in microfluidics system in vitro performed (2) small-molecule interactions with LSECs at varying concentrations assessed (3) high speed nanoscopy of LSECs within 3D endothelial tubes |